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If you want to have tight control over your assay performance and save time and money, you need to set the standard up front. The KAPA hgDNA Quantification and QC Kit provides us with a way to determine how a sample will perform in an assay and understand how the DNA is going to behave.
I would recommend the KAPA hgDNA Quantification and QC Kit to all CAP/CLIA labs and cancer testing centers because it is the most robust solution for screening both quantity and quality of sample material.
Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs.
Kapa Biosystems enzymes outperform others we’ve used. In particular, KAPA Long Range HotStart DNA Polymerase has enabled us to reliably amplify long range templates up to 12 kb for use in downstream next-generation DNA resequencing. KAPA Long Range HotStart outperformed three other competitors’ enzymes in our head-to-head comparisons. Kapa enzymes are the cornerstone of our current work and we use KAPA Long Range HotStart, KAPA2G Fast HotStart, and KAPA HiFi because of their exceptional performance and great value.
I work in a yeast genetics lab at Harvard Medical School where we had largely abandoned the use of colony PCR after finding it too unreliable – success was just too variable to consider it a useful technique. This changed after trying a sample of KAPA2G Robust HotStart DNA Polymerase. We found the success rate with this enzyme to be very high, even with difficult templates. Now, 2G Robust has become our primary enzyme, replacing a whole catalog of other polymerases that we used to keep in stock. We have again begun to use colony PCR regularly, and the only enzyme we use for it is KAPA2G Robust HotStart.
KAPA 2G Fast–the use of this second-generation polymerase allowed us to increase our throughput by 50%, while maintaining and even improving the quality of our results. This is really important because it accelerates the process of mutation detection.
I switched to the KAPA SYBR® FAST qPCR Kit from Kapa Biosystems because I noticed a significant improvement in PCR reaction efficiency … and our results more reliable.
We work with yeast and heavily rely on an efficient and reliable colony-PCR protocol to genotype our many yeast strains. After struggling with so many Taq enzymes from different brands, we found that KAPA Taq Extra is the only one working reproducibly and efficiently. On top of this, their Taq is very affordable and comes as a mix containing everything including gel loading dye. We just add our yeast, primers and you reaction is ready! Thus, for yeast colony-PCR, KAPA Taq Extra is robust, reliable, affordable, and convenient.