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Next-Generation PCR

Kapa Biosystems offers a portfolio of best-in-class PCR reagents engineered using our high-throughput molecular evolution platform. The novel DNA polymerases contained within our kits confer dramatic improvements to performance that enable results and applications not possible with wild-type enzymes.

Next-generation PCR is defined as the shift from generalist wild-type enzymes toward designer specialist enzymes. Kapa Biosystems has engineered, high performance reagents for real-time PCR, high fidelity PCR, next-generation DNA sequencing, fast PCR, hot start PCR, crude sample PCR, robust PCR, and molecular diagnostics.

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Gene Expression Analysis

Despite the high precision of qPCR, conclusions drawn from gene expression experiments are often misleading due to differences in amplification efficiency between the gene of interest and the housekeeping gene(s).

KAPA SYBR® FAST qPCR Kits contain an evolved DNA polymerase that exhibits improved speed, processivity and robustness resulting in consistently high amplification efficiencies required for accurate relative quantification, regardless of amplicon length or complexity.

For more detailed information on gene expression and how KAPA SYBR® FAST qPCR Kits can help to improve your results please download our new application notes:

Introducing KAPA Library Quantification Kits

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Eliminate time-consuming and expensive titrations and reduce variability in cluster density or template-to-bead ratio with a high performance qPCR solution for next-gen sequencing library quantification.

Standard methods for quantifying next-generation sequencing libraries have a number of important disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples, and are not suitable for high-throughput workflows.

Quantitative PCR (qPCR) is inherently well-suited for next-generation sequencing library quantification:

  • qPCR specifically quantifies only PCR-competent DNA molecules,
  • is highly sensitive allowing accurate quantification of low concentration libraries,
  • is amenable to automated liquid handling.