RNA Sequencing

RNA analysis using next-generation sequencing (RNA-Seq) enables significantly higher resolution transcriptome profiling compared to other methods such as Sanger sequencing and microarrays. RNA-Seq applications include:

  • Gene expression profiling
  • Identification of novel transcripts, isoforms and gene fusions
  • Alternative splice sites
  • Rare transcripts
  • Coding SNPs (cSNP)

Depending on the enrichment or depletion strategy, RNA-Seq can be used to evaluate different populations of RNA, including total RNA, non-coding RNA (e.g microRNA, lncRNA, etc.) and mRNA.

KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing. The kits provide precise measurement of strand orientation, uniform coverage, and high-confidence mapping of alternate transcripts.

KAPA Stranded mRNA-Seq Kits contain all the reagents in the KAPA RNA-Seq kit and the KAPA mRNA Capture Beads for the isolation of poly(A)-tailed RNA to analyze novel transcripts and isoforms, alternative splice sites, rare transcripts, and SNPs.