A reduction in PCR cycling time is an attractive proposition for most PCR users, as it allows for faster turnaround times from sample to results and improved throughput without the need for additional thermocyclers. Using wild-type enzymes, fast PCR often leads to reduced sensitivity and higher failure rates when applied across a variety of primer-template combinations.
Kapa Biosystems has successfully employed its directed evolution technology to engineer DNA polymerases specifically for fast PCR. KAPA2G Fast DNA Polymerase was evolved for higher specific activity and processivity, offering true fast PCR without loss in yield or sensitivity. Reduced total cycling times are achieved by significantly shorter extension times (1 sec/kb) than wild-type enzymes.