Consistent amplification of a broad range of targets, covering the full spectrum of AT- and GC-rich content, using a single set of reaction conditions and one cycling protocol is the ultimate goal of any lab performing routine PCR. This concept of “Single-protocol PCR” has proven impractical due to the inherent limitations of wild-type Taq DNA polymerase, most notably its inefficient amplification of challenging templates and narrow range of optimal PCR conditions. In order to improve PCR success rates and minimize reaction failures, many laboratories are forced to segregate difficult targets from “easy” targets and optimize individual reactions. The resulting subset of specialized assays disrupts workflows and increases turnaround time and cost.
To address the limitations of routine PCR, Kapa Biosystems offers products enabling the consolidation of cycling protocols, reaction conditions, and enzymes. Kapa has evolved novel, second-generation (2G) enzymes for higher processivity, offering consistent amplification, high yields and wide coverage of both easy and challenging amplicons. The versatility of the enzymes and their reaction buffers allow for the simplification of PCR workflows through the consolidation of reagents and protocols while increasing success rates and reducing turnaround times.