Genotyping refers to the process of determining genetic variations among individuals in a species. Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation and, by definition, are single-base differences at a specific locus that is found in more than 1% of the population. SNPs are often associated with human diseases and are becoming of particular interest in pharmacogenetics. Growing interest in SNP detection has been reflected by the rapid development of a diverse range of SNP genotyping methods – particularly qPCR-based protocols employing 5’- nuclease (e.g. TaqMan®) assays, FRET probes or molecular beacons.
KAPA PROBE FAST qPCR Kits are highly suitable for qPCR-based SNP genotyping using fluorescent primers or probes. KAPA PROBE FAST qPCR Master Mixes have been specifically formulated for optimal discrimination of alleles by real-time qPCR, or end-point PCR followed by allelic-discrimination analysis on a real-time instrument. In addition, KAPA PROBE FAST qPCR Master Mixes are highly stable and have been optimized for fast cycling qPCR, thus rendering these reagents well-suited for high-throughput SNP genotyping.
KAPA PROBE FAST qPCR Kits offer the following benefits for SNP genotyping:
- Discrete clusters for accurate reproducible allelic discrimination.
- Compatible with all probe-based qPCR SNP detection applications and instruments.
- Reduced cycling times to increase overall turnaround times.
- Highly stable master mixes for high-throughput workflows.