ReadyMix PCR

Kapa Biosystems offers a complete line of PCR ReadyMixes for convenience and timesaving. All ReadyMixes are supplied as 2X master mixes that contain reaction buffer, DNA polymerase, and dNTPs – just add template and primers!

KAPA2G Robust HotStart ReadyMix is recommended for increasing PCR success rates and consolidating PCR protocols. The high processivity and tolerance to common PCR inhibitors of the KAPA2G Robust DNA Polymerase allows for routine amplification of a broad range of targets (20% - 85% GC) using a single PCR protocol and reaction setup. KAPA2G Robust HotStart ReadyMix is also recommended for difficult samples, crude sample PCR, colony PCR, and applications that require high yield. Extension times of 15 sec/kb, recommended for most applications, reduces PCR cycling time by 50%.

KAPA2G Fast HotStart ReadyMix is recommended for assays that perform well with standard Taq polymerase. Reduce PCR cycling times up to 75% with the highly processive, second-generation KAPA2G Fast HotStart DNA Polymerase. Extension time as low as 1 second/kb! The 2X ReadyMix is supplied with reaction buffer, DNA polymerase, and dNTPs – just add primers and template!

KAPA HiFi HotStart ReadyMix is recommend for all high fidelity PCR applications. The KAPA HiFi DNA Polymerase is a novel proofreading polymerase with the world's lowest error rate for PCR. The high processivity of KAPA HiFi allows for increased speed and an ability to amplify long and complex targets.

KAPA Taq ReadyMix contains wild-type Taq polymerase and engineered buffer system offering high yields and consistent amplification. The 2x ReadyMix is supplied with reaction buffer, DNA polymerase, and dNTPs – just add primers and template!