Innovative solutions for next-generation sequencing library preparation.
KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing Illumina NGS libraries from fragmented dsDNA and include the following modules: End Repair, A-Tailing, Ligation, and Amplification.
Benefits of the KAPA Library Preparation Kits include:
- Higher yield of adaptor-ligated library molecules.
- Ultra pure, high quality library construction reagents supplied in convenient master mix formats.
- Reduced PCR bias resulting in improved sequencing coverage.
- Option of standard PCR library amplification module or a real-time PCR amplification module.
- Lower cost per library.
Reaction buffers are supplied in convenient, concentrated master mix formats comprising all of the required reaction components except oligonucleotide adaptors or PCR primers. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps.
The library amplification module within each KAPA Library Preparation Kit contains the KAPA HiFi HotStart DNA Polymerase, a novel enzyme engineered for high fidelity and increased affinity for DNA. The high processivity of the polymerase enables efficient amplification of targets across a broad range of GC content, minimizing bias and improving the uniformity of sequence coverage. More uniform coverage enables efficient sequencing where the highest overall coverage can be achieved from the least amount of total sequence.
KAPA Library Preparation Kits are supplied with either a standard PCR library amplification module or a real-time PCR amplification module. Real-time monitoring of library amplification provides additional information required to minimize bias due to over-amplification.