KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
KAPA T4 DNA Ligase is ideally suited for:
- routine subcloning
- recircularization of linear DNA
- library construction
- linker ligation